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Cell culture Mouse embryonic fibroblasts (MEFs) were isolated from Nrf2 +/+ , Nrf2 −/− , and Keap1 −/− mice as previously described [20] and were cultured in Iscove’s modified Dulbecco’s medium supplemented with 10% FBS and 1% penicillin–streptomycin (Invitrogen, Carlsbad, CA, USA)
AOD-9604 laboratory research
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