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Tubes were centrifuged at 300 g for 5 min at 4 °C to collect TIF
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STRENGTH:10 MG
More recently constitutive activity of expressed MOR was demonstrated using β -chlornaltrexamine ( β -CNA) in HEK293 cells (Burford, et al., 2000) and β -funaltrexamine ( β -FNA) in GH3 cells (Liu, et al., 2001) and using G protein modulation of calcium channel currents in neurons overexpressing MOR (Mahmoud et al., 2010), although it should be noted that the level of constitutive activity that has been detected is relatively small even when MOR was overexpressed
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