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Abbreviations: ME Maceration extraction SE Soxhlet extraction HPLC High Performance Liquid Chromatography MAE Microwave-assisted extraction NADES natural deep eutectic solvent USAEH ultrasound-assisted enzymatic hydrolysis TLC thin layer chromatography CE capillary electrophoresis EC electrochemical method ICH International Conference on Harmonization BPC biomass porous carbon Pt platinum CILE carbon ionic liquid electrodes MSI multispectral imaging PCA Principal Component Analysis LS-SVM Least Squares Support Vector Machine PLS Partial Least Squares ROS reactive oxygen species HAT hydrogen atom transfer BDE bond dissociation enthalpy ABTS, 2 2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) AFB1 aflatoxin B1 MDA malondialdehyde CAT catalase T-SOD total superoxide dismutase GSH-Px glutathione peroxidase T-AOC total antioxidant capacity Nrf2 Nuclearfactor erythroidderived 2-like 2 NQO1 NADH Quinone Oxidoreductase 1 HO-1 Heme Oxygenase-1 GCLC glutamate cysteine ligase catalytic ARE antioxidant response elements eNOS endothelial NO synthase Keap1 Kelch-1ike ECH- associated protein l PCOS polycystic ovary syndrome OTA ochratoxin A DXM dexamethasone GIO glucocorticoid-induced osteoporosis DOX doxorubicin TSH Thyroid-stimulating hormone LPO lipid peroxidation RONS reactive oxygen species and nitrogen XO xanthine oxidase APEC Avian pathogenic E

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Most cases in the poor group had moderate to severe misalignment, such as genus varus or valgus, or abnormal Q-angle