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Following lysis, 7.5 ml of pre-cooled QIAGEN P3 buffer was added, and cell debris was removed by sequential centrifugation (3,000 g , 15 min at 4 °C, then 15,000 g for 15 min at 4 °C) The clarified lysate was applied to QIAGEN Midiprep columns equilibrated with 10 ml of QIAGEN QBT buffer, washed with 10 ml of QIAGEN QC buffer and eluted with 5 ml of QIAGEN QF buffer

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Amount:20MG

The discovery and development of liraglutide and semaglutide