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After IEF, the IPG gel strips were equilibrated twice for 15 min under gentle shaking at room temperature, first in a solution (equilibration buffer: 50 mM Tris–HCl, pH 8.8, 6 M urea, 30% glycerol, 1% w/v SDS containing 1% dithiothreitol (DTT), then in an equilibration buffer containing 2.5% iodoacetamide
After cleansing and toning the skin
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