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Graphical Abstract Keywords Hepatitis B virus, Chronic hepatitis B, HBsAg, Immunoassay, Functional cure, Complete cure, Biomarker Introduction Despite the widespread use of the hepatitis B vaccine and antiviral drugs, chronic hepatitis B virus (HBV) infection remains a threat to human health and is the principal cause of liver cirrhosis and hepatocellular carcinoma (HCC)
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