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After centrifugation at 1000×g for 15 min, the serum levels of proBDNF and BDNF were determined using enzyme-linked immunosorbent assay (ELISA) performed on 96-well plates pre-coated with highly specific monoclonal antibodies
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This directly indicated increased tolerance to virus infection stress could be an effect of co-involvement of GSH and NPR1 which was observed in tolerant interaction of tobacco with other types of pathogens (Zechmann and Müller, 2008)
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