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The direct assessment of MDA and 4-HNE is not very sensitive, since the major portion of these reactive aldehydes is attached to proteins or other biomolecules and therefore before analysis, should be released, otherwise, they remain undetected (Esterbauer et al
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Preclinical in vivo models have examined its effects on nitric oxide synthase activity, extracellular matrix protein expression, and actin cytoskeletal dynamics in connective tissue contexts
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