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Related molecular mechanism studies using immunohistochemistry and Western blot analysis revealed significantly decreased GPX4 protein levels and upregulated pro-ferroptotic proteins ACSL4 and p53 in the OA cartilage layer
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LC–MS/MS was performed on an UltiMate 3000 nano-flow system (Dionex/LC Packings) connected to an LTQ Orbitrap hybrid mass spectrometer Velos FTMS (Thermo Fisher Scientific) equipped with a nano-electrospray ion source
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