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Biocompatible Copolymerized Gold Nanoclusters: Anti-TNF-α siRNA Binding, Cellular Uptake, Cytotoxicity, Oxidative Stress and Cell Cycle Effects In Vitro

Scenario 1: 5 mg vial + 2 mL BAC water (most common clinical setup) Vial contains: 5 mg = 5,000 mcg of BPC-157 BAC water added: 2 mL Concentration: 5,000 mcg ÷ 2 mL = 2,500 mcg/mL Per 0.1 mL (10 units on insulin syringe): 250 mcg To inject 500 mcg: 500 ÷ 2,500 = 0.2 mL (20 units) To inject 250 mcg: 250 ÷ 2,500 = 0.1 mL (10 units) Scenario 2: 5 mg vial + 1 mL BAC water (more concentrated) Vial contains: 5,000 mcg BAC water added: 1 mL Concentration: 5,000 mcg ÷ 1 mL = 5,000 mcg/mL Per 0.1 mL (10 units): 500 mcg To inject 500 mcg: 500 ÷ 5,000 = 0.1 mL (10 units) To inject 250 mcg: 250 ÷ 5,000 = 0.05 mL (5 units) Note: At this concentration, you're working with very small volumes (5 units on an insulin syringe)

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doi: 10.1101/gr.849004